The mood in the Spanish Court was grim, as King Juan's health declined further. He still was weakened and sick, but refused to withdraw from government affairs. He was content with both of his sons being present with him and helping him when he needed. He made sure to make daily confessions to safeguard his soul.
In England, Infanta Catarina of Portugal arrived on English soil. Her entourage was very grand, despite the fact that she was only marrying the brother of the King of England. She was met by her brother-in-law, Henry, Prince of Wales and she was eventually reunited with her younger sister, Joanna, Princess of Wales at Windsor Castle. The two sisters spoke in their native Portuguese did hours and all knew the two would be fast allies.
Several days later, Prince Henry, Duke of York , officially met his new bride for the first time and the forty-two year was very pleased. He was shocked to know that she also spoke English, something he understood was likely due to his youngest sister, Queen Elizabeth of Portugal. The two got along well and were married on the 25 January 1533 in St. George's Chapel. Infanta Catarina then became Catherine of Aviz, Duchess of York.
King Juan was happy when he received news from France that his daughter, Queen Catherine, had given birth to a healthy daughter on 7 February 1533. She wrote to her father that she was blessed to have a child at her age. She asked him to pray for her daughter, who was named Elisabeth.
King Alfonso asked Queen Isabel to travel to Madrid and to bring their son, Infante Juan, Prince of Viana, who was now twelve years old. They arrived on 23 March 1533. King Juan was happy to see his beautiful daughter-in-law and his grandson.
Despite his illness, King Juan made sure to negotiate in the marriage of his grandson. Queen Isabel was in favor of a match with her niece, Infanta Maria of Portugal, the eldest daughter of her brother, the King of Portugal. King Alfonso thought that a match with Naples, Florence or the Empire would do good as well. King Juan thought that keeping Iberia united was the best way and Infante Juan and Infanta Maria of Portugal were officially betrothed on 6 April 1533.
Shortly after, King Juan collapsed yet again and this time, he knew that he was was not going to get better. He was completely bedridden and this time, everyone knew that he was not going to recover. He held Council meetings from his bed and prepared to enter into the arms of his maker. However, as the weeks went by, King Juan became gravely ill to the point when he could barely speak.
King Juan was finally persuaded to withdraw from government affairs on 3 May 1533. He remained in his bed, battling high fevers And constant thirst, but did not have any appetite. All of Spain was praying for the recovery of their King, however King Juan said to pray for his soul, instead of a body that was failing. He drafted his last will and testament on 28 May 1533. He told his son to be a good King and to continue his work and to be a great King and to unite their realms. King Alfonso assured his father, through tears , that he would honor his name and Spain would be the greatest Empire on Earth.
King Juan III of Castile and Aragon died on 31 May 1533 at the age of 58 and after ruling Castile for 29 years and Aragon for 17 years. After his death, his body was embalmed and washed and dressed in fine clothes. His body was laid on a catafalque in the middle of the Chapel, where many paid respects to the late King. The new King of Spain, King Alfonso, was grief stricken, but managed to hold his composure.
The late King was placed into a coffin, covered in black cloth,with the Trastamara coat of arms on the top of his coffin. According to his wishes, King Juan ordered that his earthly remains be interred in Granada next to his wife, parents and his little daughter. Two days after his death, King Juan's funeral procession departed from Madrid, making the journey to Granada. Infante Carlos, Duke of Cadiz, was riding in the procession, fighting back tears most of the way. The King's final journey was much smoother than that of his parents' journey to Granada. Finally, on 18 June 1533, the funeral procession made it to Granada and King Juan III was laid to rest in the Royal Chapel.
His son and heir, Alfonso XII , was crowned King of Spain in Madrid on 13 July 1533. The new King was thirty years old and already a father of six children. Not long after, Queen Isabel announced her seventh pregnancy. King Alfonso wrote to his three surviving aunts, pledging his support to them, stating that his father made him promise to not let harm come to them.
In England, King Arthur was angered when the King of France repudiated the betrothal of Princess Mary and his son, Francis, Duke of Brittany and instead betrothed his son to Princess Maria of Naples. Queen Catherine tried her best to comfort her husband of thirty-two years and said they could easily find another husband for their daughter. Seventeen year old Princess Mary was not disappointed and instead joined her little sister, four year old Princess Elizabeth at Hatfield House.
On 23 November 1533, Queen Catherine of Scotland gave birth to a stillborn son. She had become pregnant several months after her marriage, but she had gone into labor three months early. The young Queen was visibly upset at the death of her first child. King James V did his best to comfort his wife and assured her that there would be more children.